• View the image of a human cheek epithelial cell in Figure 3.1.
• In lab this is what you would see under the microscope after staining the cells taken from inside your own mouth.
• The cells on this slide have been stained with methylene blue to make them easier to see.
• These cells are being viewed with the high power objective (40X) lens.
• The 3 large cheek cells are covered in many tiny bacterial cells.
• Recall in lab 2 we saw bacterial cells with a total magnification of 1000x. These have a total magnification of 400x. That is why the bacteria appear as only tiny specks on the outside of the larger cheek cells.
• Use Figure 3.1 to answer questions in Part A of the Lab Report
• Watch the video to see the differences between human and frog blood cells.
Click here to watch Human & Frog Blood Video• Use Figure 3.2 to answer questions in Part B in Lab 3 Lab Report.
• Watch the videos to understand the process of plasmolysis.
• This is a plant cell so notice the rigid cell wall and many chloroplasts.
• Use Figure 3.3 to answer questions in Part C in Lab 3 Lab Report
• Click on each button below to watch the videos to understand the Cycle Cycle & phases of mitosis.
• After watching these 3 videos you should have a good visual idea of how to distinguish the cells in different parts of the cell cycle. If you do not watch the videos you will struggle with this part of the lab.
• Use Figure 3.4 to answer questions 20-24 in Part D in Lab 3 Lab Report.
• Now that you are sure you can distinguish cells in different stages of the cell cycle see how many cells in this image are in each stage of the cell cycle.
• Use Figure 3.5 to answer questions 25 - 29 in Part D in Lab 3 Lab Report.
• NOTE - you may not see every phase. If you do not see a cell in a certain phase record the number as "0".
• NOTE- There are 40 cells in this image. Make sure you identify the phase that each of these cells is in!
• Now combine YOUR data (the answers to questions 20-24) with data collected from other lab groups and listed in Table 3.1.
• You will need to add your data (Location 1) with the data from those listed for Locations 2-4 to get the total number of cells in each phase of the cell cycle/mitosis.
• Answer questions 30 - 34 in Part D in Lab 3 Lab Report.
• Use the TOTALS obtained in the above table to answer questions 35 - 39 in Part D in your Lab 3 Lab Report.
• NOTE- If you look up the answer online you may get it incorrect. We are basing the answers to these questions on the data in Table 3.1 only!
• NOTE - Interphase is NOT a phase of mitosis. It is the phase between mitotic divisions.
Non-majors College Biology Lab Manual © 2021 by Marie McGovern Ph.D. is licensed under CC BY-NC 4.0